Articles
Analytical technology
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Title Pep-MS assay: Protease hydrolysis assay system using photo-cleavable peptide array and mass spectrometer Author M.Kurimoto, K.Shimizu*1, H.Ochi, F.Abe, and H.Honda*1,2
*1Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, *2Innovative Research Center for Preventative Medical Engineering, Nagoya UniversityJournal Journal of Bioscience and Bioengineering 128(2): 156-161(2019) Summary We have developed a "Pep-MS assay" system that could be used to comprehensively clarify the positions of protease cleavage. The Pep-MS assay can identify a lot of enzyme cleavage sites at once, and the data obtained using this technology is useful for efficiently producing bioactive peptides.