Articles

Functional Ingredients

• Title	Peptide array screening with anti-GLP-1 monoclonal antibody: Discovery of cysteine-containing DPP-IV inhibitory peptides
  Author	M.Kurimoto, N.Yuda, M.Tanaka*, M.Tanaka, and M.Okochi* *Department of Chemical Science and Engineering, Tokyo Institute of Technology
  Journal	Journal of Bioscience and Bioengineering 138(4): 351-359 (2024)
  Summary	Peptide array is a technique that allows the simultaneous evaluation of the bioactivity of a very large number of peptides. This study utilized a novel technique combining peptide arrays and antibodies to identify novel DPP-IV inhibitory peptides derived from milk proteins. Furthermore, the importance of reduced cysteine residues in DPP-IV inhibition was clarified.

• Title	Effects of casein-derived peptide Met-Lys-Pro on systolic and diastolic blood pressure: A randomized, double-blind, placebo-controlled, parallel-group study
  Author	S.Sato, D.Ochi, K.Nabeshima, R.Sakiyama, Y.Somoto, M.Nakano, M.Tanaka, and M.Nakamura* *Department of Neurosurgery, Matsumoto City Hospital
  Journal	Nutrients 16(17):2975 (2024)
  Summary	Healthy adults with high-normal and elevated blood pressure were administered a powder containing casein-derived peptide MKP for 12 weeks. The MKP group significantly decreased systolic and diastolic blood pressure compared to the placebo group.

• Title	Evaluating intestinal absorption of peptide Met-Lys-Pro in casein hydrolysate using Caco-2 and human iPS cell-derived small intestinal epithelial cells
  Author	T.Yago, N.Yuda, M.Tanaka, T.Iwao*, and T.Matsunaga* *Department of Clinical Pharmacy, Graduate School of Pharmaceutical Sciences, Nagoya City University
  Journal	Food Research International　193:114831(2024)
  Summary	The intestinal absorption of casein peptide Met-Lys-Pro (MKP) was evaluated using human iPS cell-derived small intestinal epithelial cells. As a result, transepithelial transport of MKP in casein hydrolysate exceeded that of MKP alone. These findings suggest that ingesting MKP as a casein hydrolysate may improve MKP bioavailability.

• Title	Effects of lactoferrin on oral and throat conditions under low humidity environments: A randomized, double-blind, and placebo-controlled crossover trial
  Author	S.Kubo, H.Oda, M.Tanaka, T.Koikeda*1, and S.Tomita*2 *1Shiba Palace Clinic, *2Department of Advanced Food Sciences, Faculty of Agriculture, Tamagawa University
  Journal	Nutrients 15(18): 4033 (2023)
  Summary	Healthy adults orally ingested 96.3 mg of lactoferrin or placebo followed by exposure to low humidity for 2 hours. As a result, subjective oral and throat discomfort associated with low humidity is suppressed by a ingestion of lactoferrin when compared to the ingestion of placebo.

• Title	Screening of novel DPP-IV inhibitory peptides derived from bovine milk proteins using a peptide array platform
  Author	S.Arai, M.Kurimoto, H.Nakada, M.Tanaka*, H.Ochi, M.Tanaka, and M.Okochi* *Department of Chemical Science and Engineering, Tokyo Institute of Technology
  Journal	Journal of Bioscience and Bioengineering 137(2): 94-100 (2024)
  Summary	Peptides have a variety of sequences, each of them has its own bioactivity. In this study, we identified 10 novel DPP-IV inhibitory peptides that are expected to suppress the rise in blood glucose levels, by using the "peptide array" which enables simultaneous evaluation of multiple peptide sequences. Peptide array has the potential for various applications as a method for identifying novel bioactive peptides in the future.

• Title	Allergenicity of partially hydrolyzed whey and casein formulas evaluated by ImmunoCAP inhibition assay and basophil activation test
  Author	T.Matsubara, F.Ishikawa, C.Inuo*, M.Fujita*, A.Tsukahara*, T.Koyama, H.Iwamoto, and K.Miyaji *Department of Allergy, Kanagawa Children's Medical Center
  Journal	Frontiers in Allergy, volume 4: :1207924 (2023)
  Summary	Some children develop allergic symptoms, including anaphylaxis, after the first intake of dairy product. Therefore, the tolerability of partially hydrolyzed formula (PHF), used often as a starter formula, in infants with cow’s milk allergy is important information. In this study, we used the blood of children with cow’s milk allergy and showed the tolerability of the PHFs, PHP and E-akachan, developed by our company in Japan or Indonesia is equivalent or better compared to a PHF distributed globally and characterized well.

• Title	Effects of bovine lactoferrin on the maintenance of respiratory and systemic physical conditions in healthy adults-A randomized, double-blind, placebo-controlled trial
  Author	H.Oda, S.Kubo, A.Tada, T.Yago, C.Sugita*1, H.Yoshida*1, T.Toida*2, M.Tanaka, and M.Kurokawa*1 *1Department of Biochemistry, Graduate School of Clinical Pharmacy, Kyushu University of Health and Welfare, *2School of Pharmaceutical Sciences, Kyushu University of Health and Welfare
  Journal	Nutrients 15(18):3959 (2023)
  Summary	Healthy adults ingested lactoferrin (200mg/day) or placebo for 12 weeks. As a result, the total scores for respiratory and systemic symptoms during the intervention were significantly lower in the lactoferrin group than in the placebo group. The expression of activity markers of plasmacytoid dendritic cells was significantly higher in the lactoferrin group than in the placebo group at week 12. These results suggest that orally ingested lactoferrin supports the normal immune system via maintaining the activity of plasmacytoid dendritic cells, and maintains respiratory and systemic physical conditions in healthy adults.

• Title	Whey protein hydrolysate mitigates both inflammation and endotoxin tolerance in THP-1 human monocytic leukemia cells
  Author	F.Ishikawa, T.Matsubara, T.Koyama, H.Iwamoto, ans K.Miyaji
  Journal	Immunity, Inflammation and Disease 10(12):e737 (2022)
  Summary	Severe inflammation and subsequent immunosuppression in critically ill patients is associated with a poor prognosis. In this study, we demonstrated using a human monocytic cell line that whey protein hydrolysate suppresses inflammation and endotoxin tolerance mimicking immunosuppression.

• Title	Lactoferrin and its digestive peptides induce interferon-α production and activate plasmacytoid dendritic cells ex vivo
  Author	S.Kubo, M.Miyakawa, A.Tada, H.Oda, H.Motobayashi*1, S.Iwabuchi*2, S.Tamura*3, M.Tanaka, and S.Hashimoto*2 *1Second Department of Surgery, Wakayama Medical University, *2Department of Molecular Pathophysiology, Wakayama Medical University, *3Department of Hematology/Oncology, Wakayama Medical University,
  Journal	Biometals (2022) Aug 26. Online ahead of print.
  Summary	Bovine lactoferrin and its pepsin (gastric digestive enzyme) hydrolysate enhanced interferon (IFN)-α production by peripheral blood mononuclear cells, and activated plasmacytoid dendritic cells, an important IFN-α producer, in the presence of viral single-stranded RNA

• Title	Lactulose ingestion induces a rapid increase in genus Bifidobacterium in healthy Japanese: A randomised, double-blind, placebo-controlled crossover trial
  Author	Y.Sakai, H.Ochi, and M.Tanaka
  Journal	Microorganisms 10(9): 1719 (2022)
  Summary	A clinical trial in which 36 healthy Japanese men and women consumed 4 g/day of lactulose resulted in a significant increase in bifidobacteria. The higher bifidobacterial occupancy was observed 2 days after starting lactulose ingestion and maintained until the end of lactulose intervention after 2 weeks.

• Title	Time courses of gastric volume and content after different types of casein ingestion in healthy men: A randomized crossover study
  Author	Y.Sakata, T.Yago, S.Mori*1, N.Seto, Y.Matsunaga*2, H.Nakamura, T.Tominaga*3 K.Miyaji, and Y.Takeda *1Department of Physiological Laboratory, Japanese Red Cross Sagamihara Hospital, *2Department of Sports Sciences, The University of Tokyo, *3Tominaga Internal Medicine Clinic
  Journal	The Journal of Nutrition 152(11): 2367–2375 (2022)
  Summary	We evaluated gastric emptying and plasma amino acid absorption kinetics after milk protein ingestion in a cross-over study of ten healthy men. Compared with sodium caseinate and whey protein, gastric emptying and plasma amino acid absorption kinetics after micellar casein ingestion showed slow. We also observed that the slow kinetics after micellar casein ingestion were affected by curd formation in the stomach.